2002년 국제학술지 게재논문. 한의학박사 김지훈

이 논문은 아래 논문에서와 같은 신경병증성 통증에 침치료의 효과가 일어나는 기전에 관하여 연구한 것입니다. 유전자칩을 사용하여 침치료에 의하여 특별히 발현된 유전자들을 검색하여 밝혔습니다.

이 연구는 제가 함께했던 경희대학교 연구팀과 중앙병원 연구소 연구팀이 함께 연구하였습니다.

J Biochem Mol Biol. 2002 Jul 31;35(4):420-7. 

cDNA microarray analysis of the differential gene expression in the neuropathic pain and electroacupuncture treatment models.

Ko J, Na DS, Lee YH, Shin SY, Kim JH, Hwang BG, Min BI, Park DS.

Asan Institute for Life Sciences, University of Ulsan College of Medicine 138-736, Korea. jesangko@amc.seoul.kr

Partial nerve injury is the main cause of neuropathic pain disorders in humans. Acupuncture has long been used to relieve pain. It is known to relieve pain by controlling the activities of the autonomic nervous system. Although the mechanism of neuropathic pain and analgesic effects of electroacupuncture (EA) have been studied in a rat model system, its detailed mechanism at the molecular level remains unclear. To identify genes that might serve as either markers or explain these distinct biological functions, a cDNA microarray analysis was used to compare the expression of 8,400 genes among three sample groups. Messenger RNAs that were pooled from the spinal nerves of 7 normal, 7 neuropathic pain, and 7 EA treatment rat models were compared. Sixty-eight genes were differentially expressed more than 2-fold in the neuropathic rat model when compared to the normal, and restored to the normal expression level after the EA treatment. These genes are involved in a number of biological processes, including the signal transduction, gene expression, and nociceptive pathways. Confirmation of the differential gene expression was performed by a dotblot analysis. Dot-blotting results showed that the opioid receptor sigma was among those genes. This indicates that opioid-signaling events are involved in neuropathic pain and the analgesic effects of EA. The potential application of these data include the identification and characterization of signaling pathways that are involved in the EA treatment, studies on the role of the opioid receptor in neuropathic pain, and further exploration on the role of selected identified genes in animal models.

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